ELEVATED PLUS MAZE
Elevated Plus Maze test is one of the most simple and widely used apparatus to study anxiety and anxiolytic response of almost all type of antianxiety agents on small rodents.
Exposure of the animals to novel maze alley evokes an approach-avoidance conflict which is stronger in open arm as compared to enclosed arm. It is based upon rodents (rats and mice) natural aversion for high and open space and prefer enclosed arm and, therefore, spend greater amount of time in enclosed arm.
When animals enter open arm, they freeze, become immo bile, defecate and show fear-like movements.
The plasma cortisol level is also reported to be increased, as a true reflection of anxiety.
Major advantages of this test procedure are
(a) It is simple, fast and less time consuming,
(b) No prior training or noxious stimuli (sound or light) is required.
(c) It is predictable and reliable procedure for studying anxiety response as well as antianxiety of drugs.
ELEVATED PLUS MAZE is
v Anxiety testing system for rats & mice.
v Four arms are arranged in the shape of a cross.
v Two arms have side walls and an end wall (âClosed armsâ). The two other arms have no walls (âOpen armsâ).
v Closed arm walls can be individually inserted into guide rails.
v The Opem arms are surrounded by small ledges to prevent the animal from falling from the maze.
v The maze is fastened to a light weight support frame.
v Four Infra red sensor and transmitters are fitted in each arm to monitor the entry and exit of the animal.
v The centre part of the maze is designed in such manner that whenever animal is placed on the centre table, time gets registered.
v Provided with a Control Box to monitor the activities.
v Entries in the arms are registered when mice come out.